Gram staining process | Gram staining mechanism | Gram variability reasons | Mordant & its function|

Acid, base, buffer & pH| Bronsted-Lowry concept| pKw Definition | Buffer types| Buffer capacity |

Transcription process| Abortive Transcription | Transcription termination for prokaryotes |Gre & Nus

Unit-4: Staining process | Stain Vs Dye| Chromophore & Auxochrome functions | Acidic & Basic Stain|

One shot lecture series: Bioenergetics| C.U & WBSU Semester-3| Enthalpy, Entropy & Gibbs free energy

Lineweaver-Burk Plot| Edie-Hofstee plot| Competative inhibition kinetics & curve| ATCase regulation|

Semester-3 WBSU Special|Enzyme kinetics| Establishment of Michaelis-Menten equation| Km significance

WBSU Special Molecular Biology|Transcription | Transcription unit| Bacterial promoter|RNA Polymerase

WBSU Special Lecture Series | Endospore structure | Role of Ca-DPA & SASP| Sporulation process |

Calcutta University: Semester-2 Results out 16th January| Results highlights from MicroWorld |

Mutation types|Auxotrophic & Ts Mutation | Neutral/Silent/Leaky Mutation | Flactuation test of Luria

Viral replication strategies as per Baltimore classification | phi(Φ)X174 & Retroviridae replication

RT-PCR|Real time PCR: SYBR Green & TaqMan Probe use| Sanger method of DNA Sequencing| Pyrosequencing

PCR: Theory| PCR Reaction components | PCR stages| Hot-start & Nested PCR| Inverse & Multiplex PCR|

Ti Plasmid based vector: Binary & Co-integration| Gel Electrophoresis | Blotting techniques | PAGE|

Generalised transduction mechanism | Specialised transduction | Cotansduction frequency Calculation|

Mesosome functions |Different types of inclusion bodies found in bacteria | Magnetosome| Carboxysome

Bacterial membrane structure | Fluid mosaic model| Difference in Archaebacteria membrane chemistry|

Virus life cycle| Virus entry & release strategies| Virus transmission |Virus replication strategies

Gram positive & negative bacteria Transformation steps|Experiments related to Transformation process

Bacteriophage lambda life cycle| C-I and Cro protein| Lysogenic cycle maintenance | Lytic switching|

Gene transfer methods| Transformation by CaCl2 & heat shock| Liposomal delivery | Ti plasmid & T-DNA

Tricks to solve Hfr gene mapping Problems | Bacterial transformation Introduction | Competent cell|

Bacteriophage T4 | HMC modification | T4 Replication | Terminal redundancy | Circular permutations |

Bioreacor design | Stirred tank bioreactor (STR) components & function | Air lift bioreactor |

Factors affect bacterial gene expression | Codon bias| Fungal & Insect & Mammalian expression system

Hfr Gene transfer| Hfr vs F-plasmid transfer | Interrupting mating technique |Anomalous Plateu value

Bacteriophages types| One step growth curve of viruses | Bacteriophage T4 life cycle |Eclipse period

Solid state fermentation | Submerged fermentation | Continous, Batch & Fed-batch fermentation |

Lambda vector selection | YAC Construction features | Red-White selection |Expression vector design

Microbial Genetics | Different tra genes function| Plasmid transfer mechanism | Fertility inhibition

Virus cultivation by cell culture| Bacteriophage cultivation | Assay of viruses | Viruses detection|

Industrial Microbiology | Industrial media| Carbon & Nitrogen sources | Molasses| Corn steep liquor

Bacteriophage based cloning vector | Lambda vectors- Insertional & Replacement| M13 based vector |

pBR322 & pUC Vector construction Features & selection method | Blue-White screening | IPTG & X-Gal|

R1 & F Plasmid copy no control mechanism | Conjugation discovery| Conjugative & mobilizable Plasmid|

Enzymes present in virion| Baltimore classification of viruses | Viral taxonomy | Viral cultivation|

Industrial Microbiology | Strain improvement | Feedback repression | Bypass regulatory mechanism |

Blunt end ligation | Use of linkers & Adaptors | Homopolymeric tailing| Cloning vector properties|

Plasmid curing reasons| Prevent plasmid curing| Copy no. Control of ColE1 Plasmid| Kissing complex|

Virology| Structure of viruses | Capsid symmetry | Helical & Icosahedral| Enveloped vs Naked virus|

Industrial Microbiology | Strain improvement | Site directed mutagenesis | Feedback inhibition |

Isochizomer| Star activity | DNA modifying enzymes & functions | Pol I/ RTase/ ALP/TdT/PNK functions

Semester-5 |Microbial Genetics | Plasmids partitioning systems: PAR System | Plasmid Incompatibility

Virology| Virosoids | Satellite viruses| Prions| Prions diseases| Prions replication | Pathogenesis|

Industrial Microbiology | Strain improvement strategies | Isolation of auxotroph strain| Mutation |

Semester-5 | RDT| Type-II Restriction Endonuclease | Blunt & Sticky end cutters| Restriction Mapping

Molecular Genetics | Plasmids replication | Theta mode| Rolling circle mode|Butterfly /Rabbits ear|

Semester-5 | Industrial Microbiology | Secondary screening for antibiotic | Giant colony techniques|

Semester-5 | Sub-viral particals: Viroids | Viroids structure & family | Viroids pathogenesis |

Semester-5 | Plasmids Detection by physical method & genetical method| Plasmid purification |

Industrial Microbiology | Primary screening | Crowding plate techniques | Auxanographic method|

Semester-5 | Recombinant DNA Technology | Tools Used Genetic Engineering | Restriction Endonuclease|

Semester-5| Microbial Genetics | Unit-4 Plasmids| Plasmids characteristics | Plasmids Classification

Virology | Unit-1: Nature & Properties of viruses | History of virus discovery | General Properties|

Semester-5| Industrial microbiology | Ideal Features of microbes for Industrial use|

Semester-5| Recombinant DNA Technology | Unit-1: Molecular cloning | Steps in genetic engineering |

Semester-5 Achievers Batch| Microbial Genetics | Unit-1: Nucleic acid as genetic material |CU& wbsu|

WBSU SEMESTER-1 | Protoplast & Spheroplast| Archaebacteria cell wall| Acid fast bacteria cell wall|

DNA Replication | Proofreading & Nick translation activity| Chemistry of DNA Synthesis| Termination